Václav Chochola

Experimental studies of the interplay of plasticity and magnetism in alpha-iron and chromium

Supervisor: Ing. Josef Jaroš, Ph.D.

Institution: Masaryk University

Field of Study: Anatomy, Histology and Embryolog

About my project

Recent techniques of planar cultivation and manipulation with stem/progenitor cells in vitro lack the possibilities of 3D arrangement of cells. These are crucial for creating biological models of tissues/organs with complex hierarchy and spatial combination of different cell types. Development of such tissue models can be achieved by combination of 3D bioprinting technology with functionalized hydrogels, human airway alveolar progenitors and endothelial precursors. In this project we aim to uncover conditions and factors that drive morphogenesis of highly structured (branched) organ – human lungs.

The aim is to take appropriate progenitor cells, organize them in a physiological manner using the 3D printer and let them differentiate and create lung tissue - as close to embryonic development as possible. With this, we want to study different factor that control the development.

My tasks are:

3D printing – parameter optimization, printer operation.

Cell culture of hESCs as well as differentiated progenitor cells.

Material optimization. Culturing and differentiation of cells in printed structures.

Microscopy and molecular analysis.

Where I see myself in my career in 10 years

Still as a researcher, maybe with my own small research group.

What I like most about Brno

The atmosphere. In some aspects, it is a little bit like a huge village.