Interaction of p53 family transcription factors and their modifiers with DNA in vitro and in vivo
Školitel: doc. Mons. Václav Brázda, Ph.D.
Instituce: Masaryk University, Faculty of Science
Obor: Molecular Biology
O mém projektu
Transcription factors of the p53 family play a very important role in maintaining cellular homeostasis. The p53 protein is the most mutated gene in human oncological diseases, similarly the p63 and p73 proteins play an important role in many basic cellular processes including cell cycle arrest and apoptosis. These proteins also bind to DNA and this binding to DNA can be regulated by interacting with other proteins that act either as activators or inhibitors of their transcriptional activity. Within this topic, interactions of these proteins with different structural DNA motifs will be studied using biochemical and molecular biological methods, including atomic force microscopy (AFM), flow cytometry, and in vivo in tumor cell lines using confocal microscopy.