Role of tumor microenvironment in the triple-negative breast cancer
Školitel: Mgr. Karel Souček, Ph.D.
Instituce: Masaryk University, Faculty of Science
Obor: Animal Physiology
O mém projektu
Given the heterogeneous nature of triple-negative breast cancer (TNBC) and absence of molecular targets for therapy, treatment interventions beyond conventional chemotherapy in this type of cancer remain an ongoing challenge. TNBC solid tumors are dynamic complexes, comprised of a variety of different cancer cell populations with specific signatures, which might be responsible for aggressive behavior often seen in the clinic. To address the issue of intratumoral heterogeneity and cancer plasticity we plan to introduce, optimize and validate the innovative method of mass cytometry for analysis of “TNBC cytome” in patient tissues. We believe that our setup will help identify the specific signature of selected subpopulations of cancer cells associated with plasticity and the following transcriptomic analysis might unravel new markers associated with prognosis and metastatic dissemination. In addition to pre-programmed survival mechanisms, cancer cells respond to stimuli from microenvironment and vice versa. This mutual interplay between tumor cells and their surrounding stroma can lead to support as well as suppression of tumor formation and growth. Because stromal cells can dictate the course of tumor development, identifying these stromal populations and understanding their contribution to cancer plasticity and tumor prognosis as proposed in the presented project is critical for the development of potentially useful therapeutic approaches in the future.