Single nanoparticle detection using desorption ICP MS
Školitel: Prof. Mgr. Jan Preisler, Ph.D.
Instituce: Masaryk University, Faculty of Science
Obor: Analytical Chemistry
O mém projektu
The major purpose of the project is development of instrumentation and complete methodology based on the laser ablation coupled with inductively coupled plasma mass spectrometry (ICP MS) with a single nanoparticle (NP) imaging capability from planar surfaces. As the use of the most common UV lasers may lead to NP disintegration in ablation cell, resulting in a significant loss of sensitivity, it is planned to use infrared (IR) laser at lower fluence in comparison with the UV lasers together with application of substrates and/or dyes strongly absorbing at laser emitting wavelength (1064 nm). Due to the low absorption of NPs at 1064 nm, the application of these substrate/dyes should mediate the desorption of NPs without their disintegration. Thus, the intact NPs can be introduced to ICP MS and one by one detected. The unique possibility to detect individual NPs offers many relevant applications, e.g.: NP detection in a relatively large volume in the SP mode; single NP imaging (e.g. monitoring of NP penetration in biological systems) and biomolecules imaging through selected antibody (Ab)-NPs (detection ability of single NP) and others.